Journal of Animal Science and Technology
Korean Society of Animal Science and Technology
Article

Complete genome sequence of Lactococcus taiwanensis strain K_LL004, encoding hydrolytic enzymes of plant polysaccharides isolated from grasshopper (Oxya chinensis sinuosa)

Hyunok Doo1, Hyeri Kim1, Jin Ho Cho2, Minho Song3, Eun Sol Kim1, Jae Hyoung Cho1, Sheena Kim1, Gi Beom Keum1, Jinok Kwak1, Sriniwas Pandey1, Hyeun Bum Kim1,*, Ju-Hoon Lee4,**
1Dankook University, Cheonan 31116, Korea.
2Chungbuk National University, Cheongju 28644, Korea.
3Chungnam National University, Daejeon 34134, Korea.
4Seoul National University, Seoul 08826, Korea.
**Corresponding Author: Hyeun Bum Kim, Dankook University, Cheonan 31116, Korea, Republic of. E-mail: hbkim@dankook.ac.kr.
**Corresponding Author: Ju-Hoon Lee, Seoul National University, Seoul 08826, Korea, Republic of. E-mail: juhlee@snu.ac.kr.

© Copyright 2022 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Sep 07, 2022; Revised: Oct 13, 2022; Accepted: Oct 31, 2022

Published Online: Dec 20, 2022

Abstract

The <italic>Lactococcus taiwanensis </italic>strain K_LL004 was isolated from the gut of a grasshopper (<italic>Oxya chinensis sinuosa</italic>) collected from local farm in Korea. <italic>L. taiwanensis</italic> strain K_LL004 is the functional probiotic candidate with an ability to hydrolyse plant polysaccharides. The complete genome of the <italic>L. taiwanensis</italic> strain K_LL004 contains one circular chromosome (1,995,099 bp) with a guanine + cytosine (GC) content of 38.8%. Moreover, 1,929 Protein-coding sequence, 19 rRNA genes, and 62 tRNA genes were identified based on results of annotation. <italic>L. taiwanensis </italic>strain K_LL004 has a gene, which encodes hydrolytic enzymes such as beta-glucosidase and beta-xylosidase, that hydrolyzes plant polysaccharides.

Keywords: Lactococcus taiwanensis; grasshopper; beta-glucosidase; beta-xylosidase; Whole genome sequencing