Journal of Animal Science and Technology
Korean Society of Animal Science and Technology

Comparison various level ascorbic acid and lycopene additions in semen diluent enhanced sperm quality of Sapudi ram

Sigit Bintara1,*, Dyah Maharani1, Luis Tavares2, Pradita Sitaresmi3
1Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
2Faculdade de Agricultura, Universidade nacional Timor Lorosa, Timor Leste 88111, Timor-Leste.
3Research center of Animal Husbandry, National Research and Innovation (BRIN), Bogor 16195, Indonesia.
*Corresponding Author: Sigit Bintara, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia. E-mail:

© Copyright 2023 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Mar 20, 2023; Revised: May 23, 2023; Accepted: Jun 10, 2023

Published Online: Jun 13, 2023


The primary cause of sperm quality decline during the freeze-thaw pathway is the peroxidation hazard caused by reactive oxygen species produced by the biological molecules of sperm. Ascorbic acid (Vitamin C) and lycopene are two potent antioxidants that operate to prevent oxidation processes. This study aimed to analyse the effects of ascorbic acid and lycopene on the motility, viability, abnormality and plasma membrane integrity of post-thawed Sapudi rams. Sperm samples were obtained and pooled from six sexually mature Sapudi rams, separated into ten equal proportions and diluted with TEY extender. Semen was supplemented with 0 (C0; L0), 1 (C1; L1), 2 (C2; L2), 3 (C3; L3) and 4 (C4; L4) mg/100mL (1–4%) diluent each of ascorbic acid and lycopene, respectively. Total sperm motility, viability, abnormalities and semen membrane plasma (%) were analysed after thawing. C3 and L3 extenders resulted in higher total motility (<italic>p</italic>&lt;0.05) compared to the other extenders, with all treatments higher than that of the control. The extender C3 (<italic>p</italic>&lt;0.05) exhibited the highest semen quality. Finally, the current findings show that C3 and L3 can increase the quality of post-thawed Sapudi ram spermatozoa.

Keywords: ascorbic acid; carotenoid; lycopene; Sapudi ram; vitamin C; TEY