Journal of Animal Science and Technology
Korean Society of Animal Science and Technology

Complete genome sequence of Treponema pedis GNW45 isolated from dairy cattle with active bovine digital dermatitis in Korea

Hector Espiritu1, Lovelia Mamuad1, Edeneil Jerome Valete1, Myunghwan Jung2, Sang suk Lee1, Yong il Cho1,*
1Department of Animal Science and Technology, Sunhon National University, Sucnheon 57922, Korea.
2Department of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Korea.
*Corresponding Author: Yong il Cho, Department of Animal Science and Technology, Sunhon National University, Sucnheon 57922, Korea, Republic of. Phone: +82-61-750-3234. E-mail:

© Copyright 2023 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: May 12, 2023; Revised: Jun 13, 2023; Accepted: Jun 25, 2023

Published Online: Jun 29, 2023


<italic>Treponema pedis</italic>, a fastidious anaerobic spirochete, is one of the main pathogens involved in the development and progression of bovine digital dermatitis (BDD), a lameness-causing hoof infection in cattle. Here, the complete genome sequencing of <italic>T. pedis</italic> GNW45 isolated from a dairy cow infected with BDD, was presented. Libraries for long and short reads were sequenced using PacBioRSII and Illimuna HiSeqXTen platforms, respectively. De-novo assembly was done using the long reads, producing a circular contig, by which the short reads were aligned to generate a more accurate genome sequence. The genome has a total size of 3,077,465 base pairs, with 36.84% guanine-cytosine content. A total of 2,749 protein-coding sequences, seven ribosomal RNA’s, and 45 transfer RNA’s were annotated. Functional analysis revealed genes associated with pathogenicity and survivability in the complex pathobiome of BDD. This study provided novel insights into the survival and pathogenic mechanisms of <italic>T. pedis</italic> GNW45.

Keywords: Bovine digital dermatitis; Treponema pedis; lameness; complete genome; dairy cattle