Gene | Accession or citation | Primer sequence (5’ to 3’) | Product size (bp) | Annealing temp. (°C) | |
---|---|---|---|---|---|
Satellite cell cultures | |||||
Bovine 18S rRNA1) | DQ222453 | FWD | GACACGGACAGGATTGACAG | 243 | 60.0 |
REV | CGGACATCTAAGGGCATCAC | ||||
Pax7 | ENSBTAT00000019924 | FWD | GGGCTCAGATGTGGAGTCAG | 85 | 57.0 |
REV | CATCTACACCCGAGAGGAGC | ||||
Myf-5 | ENSBTAT00000038612 | FWD | CAGCGTCTACTGTCCTGATGT | 182 | 60.5 |
REV | TTCTCAACCTGCAACTCCAG | ||||
Myogenin | ENSBTAT00000007923 | FWD | CTCCCATAGCGCCTCCTG | 162 | 60.0 |
REV | CAGACGCCCACAATCTGC | ||||
Myostatin | NM_001001525 | FWD | GTTTGGCTTGGCGTTACTCA | 178 | 60.0 |
REV | TTCCTTCTGCTCGCTGTTCT | ||||
IGF-1 | NM_001077828 | FWD | ATCACATCCTCCTCGCATCT | 131 | 60.5 |
REV | CTGTCTCCGCACACGAACT | ||||
3T3-L1 preadipocyte cultures | |||||
Bovine 18S rRNA | Granfar et al., 2005 | FWD | CTTAGAGGGACAAGTGGCG | 107 | 62.0 |
REV | ACGCTGAGCCAGTCAGTGTA | ||||
PPARγ | Taylor-Jones et al., 2002 | FWD | GCTGTTATGGGTGAAACTCTG | 351 | 60.0 |
REV | ATAAGGTGGAGATGCAGGTTC | ||||
FABP4 | Guan et al., 2002 | FWD | GGGATTTGGTCACCATCCG | 204 | 60.0 |
REV | CCAGCTTGTCACCATCTCG |