Table 1. Primer sequences used for quantification of relative gene expression of satellite cell and 3T3-L1 preadipocyte cell cultures

Gene Accession or citation Primer sequence (5’ to 3’) Product size (bp) Annealing temp. (°C)
Satellite cell cultures
 Bovine 18S rRNA1) DQ222453 FWD GACACGGACAGGATTGACAG 243 60.0
REV CGGACATCTAAGGGCATCAC
 Pax7 ENSBTAT00000019924 FWD GGGCTCAGATGTGGAGTCAG 85 57.0
REV CATCTACACCCGAGAGGAGC
 Myf-5 ENSBTAT00000038612 FWD CAGCGTCTACTGTCCTGATGT 182 60.5
REV TTCTCAACCTGCAACTCCAG
 Myogenin ENSBTAT00000007923 FWD CTCCCATAGCGCCTCCTG 162 60.0
REV CAGACGCCCACAATCTGC
 Myostatin NM_001001525 FWD GTTTGGCTTGGCGTTACTCA 178 60.0
REV TTCCTTCTGCTCGCTGTTCT
 IGF-1 NM_001077828 FWD ATCACATCCTCCTCGCATCT 131 60.5
REV CTGTCTCCGCACACGAACT
3T3-L1 preadipocyte cultures
 Bovine 18S rRNA Granfar et al., 2005 FWD CTTAGAGGGACAAGTGGCG 107 62.0
REV ACGCTGAGCCAGTCAGTGTA
 PPARγ Taylor-Jones et al., 2002 FWD GCTGTTATGGGTGAAACTCTG 351 60.0
REV ATAAGGTGGAGATGCAGGTTC
 FABP4 Guan et al., 2002 FWD GGGATTTGGTCACCATCCG 204 60.0
REV CCAGCTTGTCACCATCTCG