Table 2. Analysis of Hanwoo cow blood metabolites on different NR levels

NR group (%)	80	100	120
No. cow(Replicates of each cow)	6(18)	8(18)	5(18)
Glucose (mg/dL)	74.1±5.5	73.8±5.8	61.6±5.2
Total cholesterol (mg/dL)	126.5±3.3^c	152.6±2.4^b	177.4±1.8^a
Triglyceride (mg/dL)	24.1±1.2^b	26.5±1.3^b	34.8±1.2^a
BUN (mg/dL)	9.5±0.5^b	9.7±0.4^b	11.6±0.3^a
AST (IU/L)	69.0±2.1^b	79.8±2.0^a	75.7±2.4^a
ALT (IU/L)	21.6±0.4^c	27.2±0.5^b	30.6±0.5^a
NEFA (ЧEq/L)	440.5±18.2^a	318.5±23.1^b	195.1±8.5^c
ALB (g/dL)	3.5±0.2	3.4±0.2	3.5±0.3
TP (g/dL)	7.4±0.1^b	8.2±0.1^a	8.3±0.1^a

Blood collection was conducted after 2 h of feeding in the morning.
Blood was collected 18 times per cow, then blood metabolites were analyzed.
All values are mean ± standard error.
Values with different superscripts within a row (p < 0.05).
NR, nutrient requirements; BUN, blood urea nitrogen; AST, aspartate aminotransferase; ALT, alanine aminotransferase; NEFA, non-esterified fatty acids; ALB, albumin; TP, total protein.