Table 1. High performance liquid chromatography (HPLC) conditions for heparin analysis
Parameter
Condition
HPLC model
HP Agilent 1100, Hewlett Packard, Palo Alto, CA, USA
Column
Fortis H
2
O C
18
(150 × 4.6 mm id, 5 μm)
Mobile phase
Distilled water (DW)
Injection volume
20 μL
Flow rate
1 mL/min
Column temperature
30°C
Detector
UV 210 nm