Table 2. CCD in coded factor levels and maximum biomass as the response

Run	Treatment	Peptone soy (X1)	Yeast extract (X2)	Glucose (X3)	L-cystein (X4)	Ferrous sulfate (X5)	Maximum biomass (Y1))
1	1	−1	−1	−1	−1	−1	7.99
2	2	−1	−1	−1	−1	1	9.06
3	3	−1	−1	−1	1	−1	9.15
4	4	−1	−1	−1	1	1	9.38
5	5	−1	−1	1	−1	−1	9.68
6	6	−1	−1	1	−1	1	8.92
7	7	−1	−1	1	1	−1	9.23
8	8	−1	−1	1	1	1	9.19
9	9	−1	1	−1	−1	−1	9.35
10	10	−1	1	−1	−1	1	9.11
11	11	−1	1	−1	1	−1	10.12
12	12	−1	1	−1	1	1	9.45
13	13	−1	1	1	−1	−1	9.30
14	14	−1	1	1	−1	1	9.25
15	15	−1	1	1	1	−1	9.39
16	16	−1	1	1	1	1	9.38
17	17	1	−1	−1	−1	−1	9.17
18	18	1	−1	−1	−1	1	9.42
19	19	1	−1	−1	1	−1	9.39
20	20	1	−1	−1	1	1	9.36
21	21	1	−1	1	−1	−1	9.07
22	22	1	−1	1	−1	1	9.11
23	23	1	−1	1	1	−1	9.32
24	24	1	−−1	1	1	1	9.00
25	25	1	1	−1	−1	−1	10.29
26	26	1	1	−1	−1	1	9.18
27	27	1	1	−1	1	−1	9.45
28	28	1	1	−1	1	1	9.44
29	29	1	1	1	−1	−1	9.39
30	30	1	1	1	−1	1	9.32
31	31	1	1	1	1	−1	9.44
32	32	1	1	1	1	1	9.43
33	33	−2.366	0	0	0	0	9.03
34	34	2.366	0	0	0	0	9.30
35	35	0	−2.366	0	0	0	8.54
36	36	0	2.366	0	0	0	9.44
37	37	0	0	−2.366	0	0	9.19
38	38	0	0	2.366	0	0	9.11
39	39	0	0	0	−2.366	0	9.31
40	40	0	0	0	2.366	0	9.61
41	41	0	0	0	0	−2.366	9.16
42	42	0	0	0	0	2.366	9.18
43	43	0	0	0	0	0	9.20
44	43	0	0	0	0	0	9.34
45	43	0	0	0	0	0	9.35
46	43	0	0	0	0	0	9.31
47	43	0	0	0	0	0	9.33
48	43	0	0	0	0	0	9.16

Maximum biomass count achieved at 20 h, expressed in Log₁₀ CFU/mL.
CCD, central composite design.