| Components | BL medium (%) | Optimum medium (%) |
|---|---|---|
| Meat extract | 0.3 | - |
| Proteose peptone No.3 | 1 | - |
| Trypticase | 0.5 | - |
| Peptone | 0.3 | 28.030 |
| Yeast extract | 0.5 | 28.791 |
| Liver extract (mL) | 15 | - |
| Glucose | 1 | 6.196 |
| Soluble starch | 0.05 | - |
| Potassium phosphate buffer (mL) | 1 | - |
| K2HPO4 | 0.1 | - |
| KH2PO4 | 0.1 | - |
| Mineral mixture (mL) | 0.5 | - |
| MgSO4 | 0.001 | - |
| FeSO4 | 0.02 | 0.055 |
| NaCl | 0.000674 | - |
| MnSO4 | 0.001 | - |
| Tween 80 | 0.1 | - |
| L-Cysteine | 0.05 | 2.823 |
| Distilled water (mL) | 1,000 | 1,000 |
| Price for 250 litters of broth (USD) | 515.684 | 407.638 |
| Cost effect (%) | - | 79.04 |