Table 1. Amino acid composition (μg/mL) of the custom-made high-glucose DMEM in which MAC-T cells were incubated during all experiments at 42°C

Amino acid	Treatments1)
	CON	MET70	MET80	MET90
Composition
Lys	175	175	175	175
Met	60	70	80	90
Thr	97	97	97	97
Phe	92	92	92	92
His	74	74	74	74
Val	142	142	142	142
Ile	121	121	121	121
Leu	206	206	206	206
Arg	84	84	84	84
Trp	16	16	16	16
Ratio
Lys: Met	2.9: 1	2.5: 1	2.2: 1	1.9: 1

CON, normal amino acid profile (60 μg/mL of Met in medium); MET70, normal amino acid profile plus 10 μg/mL Met (70 μg/mL of Met in medium); MET80, normal amino acid profile plus 20 μg/mL Met (80 μg/mL of Met in medium); MET90, normal amino acid profile plus 30 μg/mL Met (90 μg/mL of Met in medium).
DMEM, Dulbecco’s modified Eagle’s medium devoid of all essential amino acids was used; MAC-T, mammary alveolar cells-large T antigen; Met, methionine; Lys, lysine; Thr, threonine; Phe, phenylalanine; His, histidine; Val, valine; Ile, isoleucine; Leu, leucine; Arg, arginine; Trp, tryptophan.