Table 4. Species richness and diversity of rumen bacteria in Holstein and Jersey steers with different levels of mineral supplementation

Parameters Holstein Jersey SEM Mixed p-value
CON NM HM CON NM HM B T B × T
ASVs 1,329.00 1,385.33 1,387.00 1,279.33 1,272.00 1,189.00 56.581 0.051 0.836 0.564
Chao1 1,332.60 1,392.55 1,391.04 1,284.44 1,275.88 1,190.71 57.306 0.052 0.823 0.562
Shannon 8.91 9.05 9.17 8.74 8.86 8.67 0.105 0.019 0.605 0.395
Inverse Simpson 0.995 0.995 0.996 0.992 0.993 0.992 0.001 0.023 0.781 0.737
Good’s coverage 1.000 1.000 1.000 1.000 1.000 1.000 0.000 0.846 0.533 0.741
Con, only TMR (without mineral supplementation); NM, TMR + NRC recommended concentration of mineral supplementation (Se 0.1 ppm + Zn 30 ppm + Cu 10 ppm)/kg DM; HM, TMR + higher than recommended concentration of mineral supplementation (Se 3.5 ppm + Zn 350 ppm + Cu 28 ppm)/kg DM; B, breed effect; T, trace mineral supplementation effect; B × T, interaction effect between breed and trace mineral supplementation.
ASV, amplicon sequence variant; TMR, total mixed rations; NRC, National Research Council; DM, dry matter.