Table 2. Cycling details of primers used for TNF alfa in this study

Cytokine Primer sequence Anneal temperature Cycles
TNF-a NM_204267 F DNA-GCC CCT GTA ACC AGA TG 57°C 40
TNF-a NM_204267 R DNA-ACA CGA CAG CCA AGT CAA CG 60.2°C 40
GAPDH NM_204305 F DNA-AGA ACA TCA TCC CAG CGT CC 58.8°C 40
GAPDH NM_204305 R DNA-CGG CAG GTC AGG TCA ACA AC 60.6°C 40
TNF, tumor necrosis factor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.