Table 2. Primers used in the quantification of the total bacteria and selected bacterial species via qPCR

Target Primer Sequence, 5ʹ to 3ʹ Annealing T (°C) Product size (bp) References
Total bacteria 27f AGAGTTTGATCMTGGCTCAG 55 1,535 [46]
1525r AAGGAGGTGWTCCARCC
Total bacteria (qPCR) Eub358f TCCTACGGGAGGCAGCAGT 60 448 [47]
Eub806r GGACTACCAGGGTATCTAATCCTGTT
Prevotella ruminicola P. rumi-F GGTTATCTTGAGTGAGTT 53 485 [48]
P. rumi-R CTGATGGCAACTAAAGAA
Clostridium aminophilum C. amin-57F ACGGAAATTACAGAAGGAAG 57 560 [49]
C. amin-616R GTTTCCAAAGCAATTCCAC
Clostridium sticklandii C. stick-185F ATCAAAGAATTTCGGATAGG 61 442 [49]
C. stick-626R CAAGTTCACCAGTTTCAGAG
Peptostreptococcus anaerobius P. anae-73F TGCTTGCAYTRATGAAAGATG 55 570 This study
P. anae-642R TCTTCCAGTTTCGGAGGCTA
qPCR, quantitative real-time polymerase chain reaction; bp, base pair.