Table 2. Top 5 significantly downregulated genes in rumen EV-treated Caenorhabditis. elegans

Gene	Gene description	Fold change (REVs/SREVs)
fbxb-43	F-box domain-containing protein	−97.29
spe-11	Spermatocyte protein spe-11	−33.41
arv-1	ARV1 homolog	−27.58
sqd-1	Homologous to Drosophila SQD (squid) protein	−17.46
Y62E10A.24	Ribonuclease P protein subunit p20	−17.07

EV, extracellular vesicle; REVs, rumen extracellular vesicles; SREVs, sonication rumen extracellular vesicles.