Table 1. Ingredient and chemical composition of the experimental diets

Item CP 20.0% CP 18.5%1)2) CP 17.0%1)2)
LP +11 AA +13 AA LP +11 AA +13 AA
Ingredient (%)
 Corn 59.35 61.86 64.28 64.28 66.06 69.38 69.38
 Soybean meal 31.75 30.60 25.46 25.46 26.35 18.76 18.76
 Soybean oil 2.50 2.50 2.50 2.50 2.50 2.50 2.50
 Corn-starch - - 0.50 - - 1.00 -
 Chromic oxide 0.50 0.50 0.50 0.50 0.50 0.50 0.50
 L-Arginine 0.12 - 0.27 0.27 - 0.44 0.44
 L-Histidine 0.00 - 0.05 0.05 - 0.11 0.11
 L-Isoleucine 0.14 - 0.23 0.23 - 0.33 0.33
 L-Leucine - - 0.04 0.04 - 0.18 0.18
 L-Lys-HCl 0.41 - 0.57 0.57 - 0.74 0.74
 L-Methionine (90% purity) 0.24 - 0.27 0.27 - 0.30 0.30
 L-Cysteine 0.15 - 0.17 0.17 - 0.20 0.20
 L-Phenylalanine - - 0.05 0.05 - 0.16 0.16
 L-Threonine 0.14 - 0.22 0.22 - 0.30 0.30
 L-Tryptophan - - 0.01 0.01 - 0.05 0.05
 L-Valine 0.17 - 0.27 0.27 - 0.37 0.37
 L-Glutamic acid - - - 0.10 - - 0.20
 L-Glycine - - - 0.40 - - 0.80
 Limestone 1.41 1.41 1.43 1.43 1.43 1.45 1.45
 Dicalcium-phosphate 1.47 1.48 1.53 1.53 1.51 1.58 1.58
 Salt 0.45 0.45 0.45 0.45 0.45 0.45 0.45
 Vitamin premix3) 0.50 0.50 0.50 0.50 0.50 0.50 0.50
 Mineral premix4) 0.50 0.50 0.50 0.50 0.50 0.50 0.50
 Choline chloride 0.20 0.20 0.20 0.20 0.20 0.20 0.20
Calculated value (%)
 AME (MJ/kg) 12.4 12.4 12.7 12.6 12.6 12.9 12.9
 CP 20.00 18.50 18.50 18.85 17.00 17.00 17.71
 Ca 0.87 0.87 0.87 0.87 0.87 0.87 0.87
 Non-phytate P 0.44 0.44 0.44 0.44 0.44 0.44 0.44
 SID AA (%)
  Indispensable AA
   Arginine 1.18 1.04 1.18 1.18 0.94 1.18 1.18
   Histidine 0.43 0.42 0.43 0.43 0.38 0.43 0.43
   Isoleucine 0.78 0.63 0.78 0.78 0.58 0.78 0.78
   Leucine 1.33 1.31 1.23 1.23 1.23 1.23 1.23
   Lysine 1.15 0.81 1.15 1.15 0.73 1.15 1.15
   Methionine 0.47 0.26 0.47 0.47 0.24 0.47 0.47
   Phenylalanine 0.77 0.76 0.73 0.73 0.70 0.73 0.73
   Threonine 0.73 0.58 0.73 0.73 0.53 0.73 0.73
   Tryptophan 0.21 0.20 0.18 0.18 0.18 0.18 0.18
   Valine 0.91 0.72 0.91 0.91 0.66 0.91 0.91
  Dispensable AA
   Alanine 0.77 0.76 0.69 0.69 0.72 0.62 0.62
   Aspartic acid 1.52 1.48 1.29 1.29 1.34 1.05 1.05
   Cysteine 0.40 0.25 0.40 0.40 0.24 0.40 0.40
   Glutamic acid 2.83 2.79 2.48 2.88 2.57 2.11 2.90
   Glycine 0.60 0.59 0.52 0.62 0.54 0.44 0.64
   Proline 1.00 0.99 0.89 0.89 0.92 0.79 0.79
   Serine 0.76 0.75 0.66 0.66 0.68 0.55 0.55
   Tyrosine 0.56 0.56 0.49 0.49 0.51 0.41 0.41
LP diet was not supplemented with AA, and overall AA were 14.5 % and 21.7% less compared to requirements in 18.5% CP and 17.0% CP diet, respectively.
LP diets supplemented with 11 AA (arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, and cysteine) were formulated to meet requirement. LP diets with 13 AA were obtained by supplementing glutamic acid and glycine to LP diets supplemented with 11 AA at the expense of corn-starch.
Supplies the following quantities per kilogram of diet: vitamin A, 31,500 IU; vitamin D3, 12,500 IU; vitamin E, 175 mg; vitamin K3, 8.75 mg; vitamin B1, 10 mg; vitamin B2, 30 mg; B5, 75 mg; vitamin B6 15 mg; vitamin B12, 0.065 mg; folic acid, 7.5 mg; niacin, 12 mg; biotin, 0.5 mg; proviox50, 10 mg.
Supplies the following quantities per kilogram of diet: Fe, 250 mg; Cu, 85 mg; Zn, 460 mg; Mn, 5,000 mg; Co, 0.75 mg; Se, 1.5 mg; I, 7.5 mg.
CP, crude protein; LP, low protein; AA, amino acid; AME, apparent metabolizable energy; P, phosphorus; SID, standardized ileal digestible.