Journal of Animal Science and Technology
Korean Society of Animal Science and Technology
Article

Complete genome sequence of probiotic Lactobacillus johnsonii 7409N31 isolated from a healthy Hanwoo calf

Young Joon Oh1, Jieun Lee2, Seul Ki Lim1, Min-Sung Kwon1, Sulhee Lee1, Sang-Pil Choi1, Dohyeon Yu3, Yeon-su Oh4, Jinho Park5,*, Hak-Jong Choi1,**
1Technology Innovation Research Division, World Institute of Kimchi, Gwangju 61755, Korea.
2Kimchi Industry Promotion Division, World Institute of Kimchi, Gwangju 61755, Korea.
3College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
4College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea.
5Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea.
**Corresponding Author: Jinho Park, Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea, Republic of. E-mail: jpark@jbnu.ac.kr.
**Corresponding Author: Hak-Jong Choi, Technology Innovation Research Division, World Institute of Kimchi, Gwangju 61755, Korea, Republic of. Phone: +82-62-610-1729. E-mail: hjchoi@wikim.re.kr.

© Copyright 2022 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Aug 24, 2022; Revised: Oct 20, 2022; Accepted: Oct 31, 2022

Published Online: Dec 20, 2022

Abstract

Lactobacillus johnsonii 7409N31 was isolated from the feces of a healthy 11-day-old Hanwoo calf from a farm in Geochang-gun, Gyeongsangnam-do, Korea. The genome of the strain was completely sequenced using the PacBio RSII sequencing system, and it was confirmed that it was composed of one circular chromosome. The size of the entire genome was 2,198,442 bp, and it had 35.01 mol% G + C content and 2,222 protein-coding sequences, 24 rRNA, 3 ncRNA, and 112 tRNA genes. Strain 7409N31 possessed genes encoding enzymes involved in the hydrolysis of both fibrous and non-fibrous carbohydrates. These data provide a comprehensive theoretical understanding for developing industrial probiotic feed additives that improve nutrient digestibility.

Keywords: Lactobacillus johnsonii; Complete genome sequence; Hanwoo calf; Probiotics