Table 1
Results of determination of ribonucleolytic activity (A, OD260) remained in the supernatant liquids after centrifugation of the heat-treated enzyme samples
ToC | Ribonucleolytic activity remained after heat treatment |
---|
Series 1 | Series 2 | Series 3 | Series 4 | Series 5 | Series 6 |
---|
OD260 | % | OD260 | % | OD260 | % | OD260 | % | OD260 | % | OD260 | % |
---|
25 | 0.165 | 100.0 | 0.206 | 100.0 | 0.190 | 100.0 | 0.166 | 100.0 | 0.229 | 100.0 | 0.228 | 100.0 |
30 | 0.214 | 129.3 | 0.254 | 123.6 | 0.239 | 125.9 | 0.193 | 115.9 | 0.259 | 113.0 | 0.250 | 109.5 |
40 | 0.227 | 137.0 | 0.270 | 131.6 | 0.257 | 135.0 | 0.212 | 127.4 | 0.279 | 121.7 | 0.284 | 124.6 |
50 | 0.239 | 144.5 | 0.289 | 140.7 | 0.262 | 137.9 | 0.224 | 135.2 | 0.297 | 129.2 | 0.299 | 131.4 |
60 | 0.206 | 124.2 | 0.276 | 134.3 | 0.254 | 133.5 | 0.215 | 129.4 | 0.252 | 109.8 | 0.274 | 120.1 |
70 | 0.200 | 121.0 | 0.241 | 117.2 | 0.215 | 113.2 | 0.189 | 113.8 | 0.221 | 96.1 | 0.231 | 101.4 |
80 | 0.174 | 105.3 | 0.234 | 113.6 | 0.205 | 107.6 | 0.176 | 105.9 | 0.209 | 91.0 | 0.204 | 89.4 |
90 | 0.169 | 102.4 | 0.223 | 108.7 | 0.203 | 106.7 | 0.166 | 99.9 | 0.203 | 88.7 | 0.198 | 87.1 |
100 | 0.155 | 93.5 | 0.212 | 103.2 | 0.170 | 89.4 | 0.146 | 88.1 | 0.197 | 85.7 | 0.186 | 81.6 |