Table 1. Composition (%, as-is) of the experimental diets

Total lysine (%) Treatments
0.55 0.59 0.63 0.67 0.71 0.75 0.79
Daily lysine intake (mg/d) 732 785 838 891 944 997 1,050
 Corn - - 6.80 24.00 48.70 48.70 48.70
 Wheat 67.88 67.88 60.88 38.36 11.16 10.16 10.10
 Wheat bran 13.39 11.98 10.66 13.00 12.57 12.94 12.94
 Soybean meal 7.36 9.20 10.70 13.47 16.40 17.00 17.00
 Vegetable oil 1.93 1.50 1.50 2.00 2.00 2.00 2.00
 Limestone 6.70 6.70 6.70 6.60 6.60 6.60 6.60
 Monocalcium phosphorous 1.80 1.80 1.80 1.80 1.80 1.80 1.80
 Salt 0.30 0.30 0.30 0.30 0.30 0.30 0.30
 Vitamin-mineral premix1) 0.30 0.30 0.30 0.30 0.30 0.30 0.30
 L-Lysine - - 0.02 - - 0.03 0.09
 DL-Methionine 0.17 0.17 0.17 0.17 0.17 0.17 0.17
 L-Threonine 0.17 0.17 0.17 - - - -
Calculated composition2)
 ME (kcal/kg) 2,800 2,800 2,800 2,805 2,807 2,800 2,800
 Crude protein (%) 15 15 15 15 15 15 15
 Calcium (%) 3.00 3.00 3.00 3.00 3.00 3.00 3.00
 Available P (%) 0.50 0.50 0.50 0.50 0.50 0.50 0.50
 Total lysine (%) 0.55 0.59 0.63 0.67 0.71 0.75 0.79
Vitamin-mineral premix provided following nutrients (per kg of air-dry diet): Vitamins: A 12,000 IU, D 33,000 IU, E 15 mg, K 2 mg, thiamine 2 mg, riboflavin 6 mg, pyridoxine 2 mg, calcium pantothenate 0.03 mg, folic acid 0.2 mg, niacin 45 mg, biotin 0.15 µg. Minerals: calcium 0.5%, Co 0.5 mg (as cobalt sulphate), Cu 10 mg (as copper sulphate), iodine 0.9 mg (as potassium iodine), iron 80 mg (as ferrous sulphate), Mn 80 mg (as manganous oxide), Se 0.2 mg (as sodium selenite), Zn 80 mg (as zinc oxide).
The values are calculated according to the values of feedstuffs in NRC [2].
ME, metabolizable energy.