Table 1. Ingredient composition and calculated composition of the basal diets for manufacturing the experimental diets1)

PC_1-3 PC_4-5 NC_1-3 NC_4-5 ENC_1-3 ENC_4-5
Ingredients (%)
 Corn 48.51 60.96 48.96 62.93 48.64 62.84
 Wheat 8.40 4.12 10.09 3.68 11.42 5.63
 Wheat bran 4.10 - 3.70 - 4.30 -
 SBM 48% 31.15 27.62 29.60 26.37 27.98 24.70
 Vegetable oil 3.30 3.30 3.00 3.00 3.00 2.80
 Limestone 1.20 0.95 1.20 0.96 1.20 0.97
 Monocal P 1.65 1.39 1.70 1.39 1.70 1.39
 Salt 0.30 0.35 0.35 0.35 0.35 0.35
 Vitamin-mineral premix2) 0.30 0.30 0.30 0.30 0.30 0.30
 Lysine-HCl 0.34 0.30 0.36 0.31 0.37 0.33
 DL-Methionine 0.20 0.19 0.19 0.18 0.19 0.17
 L-Threonine 0.13 0.11 0.13 0.12 0.13 0.11
 L-Cystine 0.12 0.11 0.12 0.11 0.12 0.11
 Chromium oxide powder (Cr2O3, maker)3) 0.30 0.30 0.30 0.30 0.30 0.30
Calculated composition4)
 Metabolizable energy (kcal/kg) 3,060 3,200 3,060 3,200 3,060 3,200
 Crude protein (%) 21.50 19.50 21.00 19.00 20.50 18.50
 SID lysine (g/MJ ME) 0.94 0.80 0.92 0.79 0.90 0.77
Calculated SID amino acids (%)
 Lysine 1.20 1.08 1.18 1.05 1.15 1.03
 Methionine 0.48 0.45 0.46 0.44 0.46 0.42
 Cystine 0.43 0.40 0.43 0.39 0.42 0.39
 Threonine 0.82 0.74 0.80 0.73 0.78 0.70
 Tryptophan 0.22 0.19 0.21 0.18 0.20 0.18
 Iso-leucine 0.75 0.68 0.72 0.66 0.70 0.64
 Leucine 1.56 1.49 1.52 1.46 1.48 1.42
 Valine 0.85 0.78 0.82 0.76 0.80 0.73
 Histidine 0.54 0.49 0.52 0.48 0.51 0.46
 Arginine 1.25 1.10 1.20 1.06 1.16 1.01
 Methionine + cystine 0.91 0.85 0.89 0.83 0.88 0.81
The eight dietary treatments were: positive control (PC), negative control (NC: minus 0.5% from PC, and minus 2% of lysine, methionine, threonine and methionine plus cysteine), extreme negative control (ENC: minus 1% from PC, minus 4% of lysine, methionine, threonine and methionine plus cysteine), and plus multi-protease 150 or 300 g per ton (e. g., PC-150); PC, PC-150, NC, NC-150, NC-300, ENC, ENC-150, ENC-300.
Provided per kilogram of diet: vitamin A, 12,000 IU; vitamin D3, 2,500 IU; vitamin E, 30 IU; vitamin K3, 3 mg; D-pantothenic acid, 15 mg; nicotinic acid, 40 mg; choline, 400 mg; and vitamin B12, 12 μg; Fe, 90 mg from iron sulfate; Cu, 8.8 mg from copper sulfate; Zn, 100 mg from zinc oxide; Mn, 54 mg from manganese oxide; I, 0.35 mg from potassium iodide; Se, 0.30 mg from sodium selenite.
Chromium oxide powder (Cr2O3: > 99.9% purity, Sigma-Aldrich, USA).
The values were calculated according to the values of feedstuffs [20].
SBM, soybean meal; SID, standardized ileal digestible.