Table 1. Primers used for quantitative real-time PCR analysis

Gene (GenBank ID)	Forward primer sequence	Reverse primer sequence	Tm (°C)	Expected PCR size (bps)
Star (NM_031558)	CTGCTAGACCAGCCCATGGAC	TGATTTCCTTGACATTTGGGTTCC	66	91
Cyp11a1 (J05156)	AGGTGTAGCTCAGGACTT	AGGAGGCTATAAAGGACACC	52	399
Cyp17 (M31681)	AGATTGACCAGTACGTAGGCTTCAGCCGAA	CACATCCAAGTCAAACCTCTGCAGTAGC	67	410
Hsd3b1 (M38178)	CCCATACAGCAAAAGGATGG	GCCGCAAGTATCATGACAGA	55	150
Hsd17b3 (NM_054007.1)	CTCCCAACCTGCTCCCAAGTCATTT	AGCAAGGCAGCCACAGGTTTCAGC	65	408
Cyp19 (M33986)	GCTTCTCATCGCAGAGTATCCGG	CAAGGGTAAATTCATTGGGCTTGG	62	290
Ppia (NM_017101)	GGCAAATGCTGGACCAAACAC	TTAGAGTTGTCCACAGTCGGAGATG	62	196

PCR, polymerase chain reaction; PCR, polymerase chain reaction; Star, steroidogenic acute regulatory protein; Cyp11a1, cytochrome P450 side chain cleavage; Cyp17, cytochrome P450 17a-hydroxylase; Hsd3b1, 3β-hydroxysteroid dehydrogenase/delta-5-delta-4 isomerase type I; Hsd17b3, 17β-hydroxysteroid dehydrogenase 3; Cyp19, cytochrome P450 aromatase; and Ppia: cyclophilin A.