Table 2. Primer sequences for chicken PPDPF cloning

Purpose	Sequences (5’ to 3’)	Accession No.
Isolation	F: CCAGGTTTTCCATCAGCACAA	NM_001197037.1
	R: AAGGGAAGGGCCATTGCAG
Cloning	F: AAGCTTATGGCCTCCATCCCATCGAGC	NM_001197037.1
	R: GGATCCGGATGAGTGCCCAATGCCTGG

Primers for isolation of PPDPF were in outside of CDS.
Primers for cloning containing HindIII (AAGCTT) or BamHI (GGATCC) sequences.
PPDPF, progenitor cell differentiation and proliferation factor; CDS, coding sequences; F, forward; R, reverse.