Table 3. Primer sequences specific for the target genes used for real time PCR
Gene
Accession number
1)
Sequence (5′ to 3′)
Length (bp)
HSPB6
NM_001076027
F: GGTGTTGCTGGATGTGAAAC
R: GCAATGTATCCGTGCTCATC
117
PPARγ2
Y12420
F: CATAATGCCATCAGGTTTGG
R: GTCAGCAGACTCTGGGTTCA
102
Desmin
NM_001081575
F: GGACCTGCTCAATGTCAAGA
R: GGAAGTTGAGGGCAGAGAAG
109
Beta-actin
NM_173979
F: GCGTGGCTACAGCTTCACC
R: TTGATGTCACGGACGATTTC
54
Database protein names and accession numbers: NCBI (
http://www.ncbi.nlm.nih.gov
).
PCR, polymerase chain reaction; bp, base pair; HSPB6, heat shock protein beta-6; PPARγ, peroxisome proliferator-activated receptor gamma.