Table 4. Characterization of candidate proteins affected caloric restriction in Korean native steers

Spot1)	Name2)	Accession number2)	MW / pI3)	Score	Sequence coverage (%)	Spot intensity		Fold change4)
						Normal	Restricted
1	Inner membrane protein, mitochondrial	A7E3V3	83.05 / 6.37	51.03	31.38	0.01	0.03	0.47
2	SuLFEotransferase, estrogen-preferring	P19217	34.62 / 6.67	72.35	20.00	0.03	0.07	0.44
3	Myosin 1	Q9BE40	22.29 / 5.57	102.41	14.50	0.25	0.10	2.44
4	Alpha-1-antiproteinase precursor	P34955	46.10 / 6.05	14.64	16.35	0.22	0.08	2.68
5	Alpha-1 antiproteinase	P34955	46.10 / 6.05	4.55	4.81	0.19	0.08	2.32
6	Chain Q, Ca model of bovine Tric CCT devrived from A 4.0 AnGSTROM	3IYG_Q	55.77 / 5.33	27.62	22.85	0.05	0.10	0.46
7	Apolipoprotein H	P17690	38.25 / 8.53	47.64	50.63	0.38	0.80	0.48
8	Heat shock protein beta-1	Q3T149	22.40 / 5.98	22.18	40.80	0.39	0.82	0.47
9	Heat shock protein beta-6	Q148F8	17.47 / 5.95	15.43	47.56	0.20	0.43	0.47

The spot number refers to Fig. 1.
Database protein names and accession numbers: UniProt (www.uniprot.org).
Molecular weight (MW) and isoelectric point (pI) of each protein were determined by 2-DE.
The expression ratios of spot intensity at ad libitum group versus caloric restriction group.