Table 1. Composition of the experimental diets (%, as-fed basis)

Ingredient (%) Diets1)
3200 3100 3000 2900 2800 2700
  Corn 42.55 38.04 33.53 29.02 24.51 20.00
  Wheat HRW 24.00 22.20 20.40 18.60 16.80 15.00
  Wheat bran - 4.90 9.80 14.70 19.60 24.50
  SBM (48%) 30.00 29.20 28.40 27.60 26.80 26.00
  Oats - 2.00 4.00 6.00 8.00 10.00
  Vegetable oil 0.20 0.41 0.62 0.83 1.04 1.25
  Limestone 1.00 1.00 1.00 1.00 1.00 1.00
  Monocal phos Biofos 1.50 1.50 1.50 1.50 1.50 1.50
  Salt 0.30 0.30 0.30 0.30 0.30 0.30
  Vitamin premix2) 0.30 0.30 0.30 0.30 0.30 0.30
  DL-methionine 0.15 0.15 0.15 0.15 0.15 0.15
Calculated composition
  ME (kcal/kg) 3,203.0 3,103.2 3,003.4 2,903.6 2,803.8 2,704.0
  CP (%) 21.11 21.11 21.11 21.12 21.12 21.12
  Lys (%) 1.11 1.11 1.10 1.10 1.09 1.09
  Met + Cys (%) 0.85 0.85 0.85 0.86 0.86 0.86
Teatment number indicate dietary AME (kcal/kg).
Vitamin and mineral pre-mixture provided the following nutrients per kg of diet: vitamin A, 24,000 IU; vitamin D3, 6,000 IU; vitamin E, 30 IU; vitamin K, 4 mg; thiamine, 4 mg; riboflavin, 12 mg; pyridoxine, 4 mg; folacine, 2 mg; biotin, 0.03 mg; vitamin B8 0.06 mg; niacin, 90 mg; pantothenic acid, 30 mg; Fe, 80 mg (as FeSO4 ・H2O); Zn, 80 mg (as ZnSO4 ・ H2O); Mn, 80 mg (as MnSO4 ・H2O); Co, 0.5 mg (as CoSO4 ・H2O); Cu, 10 mg (as CuSO4 ・ H2O); Se, 0.2 mg (as Na2SeO3); I, 0.9 mg (as Ca [IO3] ・2H2O).
HRW, hard red winter; SBM, soybean meal; ME, metabolizable energy; CP, crude protein; Met, methionine; Lys, lysine; Cys, cysteine; AME, apparent metabolizable energy.