Table 3. Primer pairs, accession number, annealing temperature, and product sizes of primers used in RT-PCR reaction

Gene	F/R	Sequence (5’ → 3’)	Accession number	Annealing temperature	Length
Beta-casein	Forward	AAATCTGCACCTTCCTCTGC	XM_015471671.2	59.0°C	123
Beta-casein	Reverse	GAACAGGCAGGACTTTGGAC	XM_015471671.2	59.0°C	123
S6K1	Forward	GGACATGGCAGGGGTGTTT	XM_027519044.1	55.6°C	138
S6K1	Reverse	GGTATTTGCTCCTGTTACTT	XM_027519044.1	55.6°C	138
Beta-actin	Forward	GCATGGAATCCTGCGGC	NM_173979	58.0°C	121
Beta-actin	Reverse	GTAGAGGTCCTTGCGGATGT	NM_173979	58.0°C	121

RT-PCR, real-time polymerase chain reaction; S6K1, S6 kinase beta-1.