Table 6. Differentially expressed proteins upon supplementation with D-Met and HMBi in MAC-T cells compared to control

Detection protein	D-Met	HMBi
Increasing number	46	40
Decreasing number	68	78
Selected downregulated and upregulated proteins
Eukaryotic translation initiation factor 3 subunit A (EIF3A)		▲
Ribosomal protein S21 (RPS21)		▼
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	▲
Malate dehydrogenase, mitochondrial (MDH2)	▲	▲
Eukaryotic translation initiation factor 4A1 (EIF4A1)	▼
Eukaryotic translation initiation factor 4A2 (EIF4A2)	▲
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1 (ATP5A1)
Ribosomal protein S12 (RPS12)		▼

▲, Upregulated (> 2-fold protein expression than in control); ▼, Downregulated (< 0.5-fold protein expression than in control).
D-Met, D-methionine; HMBi, 2-hydroxy-4-methylthiobutanoic acid I; MAC-T, immortalized bovine mammary epithelial cell line.