| Invasiveness of sampling | Low [77] | Low [77] |
| Risk of contamination from external sources | Yes [78] | No |
| Training required for sampling | No | Yes |
| Sampling procedure may confound results | No | No |
| Possibility of repeated sampling | Yes | No |
| Ethical and legal licenses required | No [77] | Yes [79] |
| Biosecurity concerns | Yes | Yes |
| Results depend on time of sampling | Yes | No |
| Results depend on sampling location | No | No |
| Steroid medications may confound results | Yes | No |
| Sampling requires live animals | Yes | Yes |
| Sample preparation is laborious and time consuming | No | Yes |
| Storage conditions | Refrigerator, freezer | Refrigerator, freezer |
| Time range represented by biomarker measurement | 2–4 days | Week/month |
| Biomarker forms represented | Unbound | Unbound |
| Stress type | Acute | Chronic [77] |
| Analytical cost | Relatively inexpensive | Relatively inexpensive |