Table 5. Properties of solid biomatrices with regard to measurement of cortisol and corticosterone

Properties Solid biomatrix
Hair Feathers Nails/claws/ hooves Scales
Invasiveness of sampling Low [82,83] Low Low [71,84] Low [71,85]
Risk of contamination from external sources Yes Yes No No
Training required for sampling procedure No [57] No [40] No No
Sampling procedure may confound results No No No No
Possibility of repeated sampling Yes Yes Yes No
Ethical and legal licenses required Yes Yes [40] No Yes [85]
Biosecurity concerns Low Low [86] Low Low
Results depend on time of sampling No [87] No [40] No No
Results depend on sampling location Yes [57] Yes No No
Steroid medications may confound results No No No No
Sampling requires live animals No No [86] No No
Sample preparation is laborious and time consuming Yes Yes Yes Yes
Storage conditions Room temperature [87] Room temperature [40] Room temperature [84] Refrigerator, freezer [85]
Time range represented by biomarker measurement Week, month, year Week, month, year Week, month, year Week, month, year
Biomarker forms represented Unbound [88] Unbound [88] Unbound [88] Unbound [88]
Stress type Chronic [82,83] Chronic [67,86] Chronic [71,84] Chronic [71,85]
Analytical cost Relatively inexpensive [72] Relatively inexpensive [40] Relatively inexpensive Relatively expensive