Table 2. Influence of DON on expression of tight junction proteins in epithelial cells and pigs intestine tissue

Study	Samples	DON concentration	Effects	Reference
In vitro	IPEC-1	0.2 to 2 µM	Reduction of ZO-1 protein in higher concentration	[7]
	IPEC-J2	0.2 to 2 µM	Reduction of ZO-1 protein in higher concentration	[7]
	IPEC-1	10, 30 µM	Claudin-3,4 reduced at high concentration of DON	[52,56]
	IPEC-J2	2 µM	Decreased ZO-1, occludin and claudin-3 expressions	[57]
In vivo	Pigs jejunum	2.85 mg/kg feed	40% decrease of claudin-4 expression	[52]
	Pigs ileum	3.5 mg/kg feed	mRNA expression of claudin-3 and 4 and occluding were decreased	[58]
	Pigs jejunum	1.006 mg/kg feed	Decreased claudin-4 expression	[13]
	Pigs ileum	3 mg/kg feed	Decreased expression of E-cadherin and occluding junction proteins	[43]

DON, deoxynivalenol; IPEC-1, intestinal porcine epithelial cell line-1.