Table 2. Primers used for the gene expression analysis of growing cattle-derived intestinal organoids

Gene name	Forward	Reverse
18S rRNA	AAACGGCTACCACATCCAAG	CCTCCAATGGATCCTCGTTA
LGR5	GTGTTCAGAGCCGCAGTGTA	GATTCCGAAGCAAAAATGCA
Bmi1	TTCATTGATGCCACAACCAT	CAGCATCAGCTGAAGGATGA
CDX2	CATCACCTCACCACCATCC	GTTTTCACTTGGCTGCCCAG
SOX9	TTCATGAAGATGACCGACGA	GTCCAGTCGTAGCCCTTGAG
FOXA3	GCAAGATGCTTACCCTGAGC	AGGTAGCAGCCGTTCTCAAA
HNF4A	CGAGCAGATCCAGTTCATCA	GAAGGCTGTGGAGTCTCAGG
MUC2	TTCGACGGGAGGAAGTACAC	TTCACCGTCTGCRTCATTCAG
E-cadherin	CCAGGTGACCACACTTGATG	ATACACATTGTCCCGGGTGT
Chromogranin A	TATCAATCCTGCGACATCAG	CTGTCTCCGTCCGAGTCTTC