Table 2. Quantitative real-time polymerase chain reaction primer design

Target group/specificity	Primer sequence
Universal	Forward	GTGSTGCAYGGYYGTCGTCA
Universal	Reverse	ACGTCRTCCMCNCCTTCCTC
Bacteroides spp.	Forward	GAAGGTCCCCCACATTG
Bacteroides spp.	Reverse	CGCKACTTGGCTGGTTCAG
Roseburia spp. and E. rectale	Forward	GCGGTRCGGCAAGTCTGA
Roseburia spp. and E. rectale	Reverse	CCTCCGACACTCTAGTMCGAC
Faecalibacterium prausnitzii	Forward	GGAGGAAGAAGGTCTTCGG
Faecalibacterium prausnitzii	Reverse	AATTCCGCCTACCTCTGCACT
Cluster IV Ruminococcus spp.	Forward	GGCGGCYTRCTGGGCTTT
Cluster IV Ruminococcus spp.	Reverse	CCAGGTGGATWACTTATTGTGTTAA
Bifidobacterium spp.	Forward	TCGCGTCYGGTGTGAAAG
Bifidobacterium spp.	Reverse	GGTGTTCTTCCCGATATCTACA
Methanogens	Forward	GGATTAGATACCCSGGTAGT
Methanogens	Reverse	GTTGARTCCAATTAAACCGCA
Oscillospira spp.	Forward	ACGGTACCCCTTGAATAAGCC
Oscillospira spp.	Reverse	TCCCCGCACACCTAGTATTG
Leuconostoc mesenteroid	Forward	TGATGCATAGCCGAGTTGAG
Leuconostoc mesenteroid	Reverse	GAAAGCCTTCATCACACACG
Leuconostoc citreum	Forward	GGAAACAGATGCTAATACCGAATA
Leuconostoc citreum	Reverse	TTTACCCCACCAACTAACTAATG
Weissella cibaria	Forward	GGGAAACCTACCTCTTAGCA
Weissella cibaria	Reverse	GGACCATCTCTTAGTGATAGCA
Weissella koreensis	Forward	GGGCTACACACGTGCTACAA
Weissella koreensis	Reverse	GATTCCGACTTCGTGTAGGC
Lactobacillus sakei	Forward	CCATGTGTAGCGGTGAAATG
Lactobacillus sakei	Reverse	ATCCTGTTTGCTACCCATGC

Adapted from Da Yoon Yu et al. with CC-BY-NC [6].