Table 3. Reverse transcriptase polymerase chain reaction primer design

Target group/specificity	Primer sequence
TGF-β	Forward	GAAGGCAGAGTTCAGGGTCTT
TGF-β	Reverse	GGTTCCTGTCTTTGTGGTGAA
IL-10	Forward	ATAACTGCACCCACTTCCCA
IL-10	Reverse	TCATTTCCGATAAGGCTTGG
IFN-γ	Forward	TCAAGTGGCATAGATGTGGAAGAA
IFN-γ	Reverse	TGGCTCTGCAGGATTTTCATG
IL-17A	Forward	TTCATCTGTGTCTCTGATGCT
IL-17A	Reverse	TTGACCTTCACATTCTGGAG
IL-4	Forward	ACAGGAGAAGGGACGCCAT
IL-4	Reverse	GAAGCCCTACAGACGAGCTCA
Foxp3	Forward	CCCATCCCCAGGAGTCTTG
Foxp3	Reverse	CCATGACTAGGGGCACTGTA
GATA3	Forward	CATTACCACCTATCCGCCCTATG
GATA3	Reverse	CACACACTCCCTGCCTTCTGT
T-bet	Forward	TCAACCAGCACCAGACAGAG
T-bet	Reverse	AAACATCCTGTAATGGCTTGTG
RORγt	Forward	TTCACCCCACCTCCACTG
RORγt	Reverse	TGCAAGGGATCACTTCAATTT

Adapted from Da Yoon Yu et al. with CC-BY-NC [6].
TGF-β, transforming growth factor beta; IL-10, interleukin-10; IFN-γ, interferon-γ; Foxp3, Forkhead box P3; GATA3, GATA binding protein 3; T-bet, T-box expressed in T cells; RORγt, RAR-related orphan receptor gamma T.