Table 1. The primer sequences used for quantitative real-time PCR of muscle and adipose tissue

Gene	Primer sequence	Temp (°C)	Time (s)
GAPDH	F: CTCAACGGGAAGCTCACTGG	55.9	30
GAPDH	R: TGTCGTACGAGGAAATGAGC	55.9	30
Pax 3	F: GCAGCACCGTTCACAGACCT	57.9	30
Pax 3	R: CGGGGTTCATGGGGTTGGAG	57.9	30
MyoD	F: TGCGTATTCTCAACCCCTTC	53.8	30
MyoD	R: AGTATGCAAGGGTGGAGTGG	53.8	30
Myogenin	F: GTGAATGAGGCCTTTGAGGC	53.8	30
Myogenin	R: TGTGGGAACTGCATTCACTG	53.8	30
MHC	F: TCAAGGGGAGATCACTGTCC	53.8	30
MHC	R: TCAGCAACTTCTGTGCCATC	53.8	30
MSTN	F: TCTCGATGCTGTCGTTACCCT	59.9	30
MSTN	R: GCACCAAGCAAACCCCAGA	59.9	30
PLAG1	F: ACCCGTTCGGTTCTACCTCA	53.8	30
PLAG1	R: TTAGACGACGACGCTGGAGA	53.8	30
FASN	F: GTCCTGCTGAAGCCTAACTC	53.8	30
FASN	R: TCCTTGGAACCGTCTGTG	53.8	30
CEBP-β	F: TGTGTACAGATGAATGATAAACTCTGC	55.2	30
CEBP-β	R: GGTTTCGAAGTTGATGCAATC	55.2	30
SCD	F: CTACACAACCACCACTACCATCAC	57.4	30
SCD	R: GCAAACGCCCAGAGCAAGG	57.4	30
PPAR-γ	F: ATGGGTGAAACTCTGGGAGA	53.8	30
PPAR-γ	R: TCAAAGGAGTGGGAGTGGTC	53.8	30
Adiponectin	F: TCCACGTCACGGTCTACTTG	53.8	30
Adiponectin	R: TTCTCTTCATCCCCGTATGC	53.8	30

PCR, polymerase chain reaction.