Table 1. Primer sequences for DF-1 chicken embryonic fibroblasts

Gene	Sequence (5′ to 3′)	Accession No.	Temperature (℃)	Product size (bp)
IFI6	F: GCCGGTTTCACTTCCTCTGGR: CCCCCAAAGGATTTTGCCTC	NM_001001296.6	60	80
INF-α	F: GACAGCCAACGCCAAAGCR: GTCGCTGCTGTCCAAGCATT	NM_205427.1	60	342
IRF7	F: GAGGATCCGGCCAAATGGAAR: CCAAATCGTGGTGGTTGAGC	NM_205372.2	60	211
GAPDH	F: TGCTGCCCAGAACATCATCCR: ACGGCAGGTCAGGTCAACAA	NM_204305.2	60	142

bp, base pair; IFI6, interferon-alpha inducible factor 6; IFN-α, interferon-alpha; IRF7, interferon regulatory factor 7; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.