| Microflora | Primer sequence | Anneal/extension temperature | Cycles |
|---|---|---|---|
| F:DNA-AGC AGT AGG GAA TCT TCC A | 54 | 40 | |
| R:DNA-CAC CGC TAC ACA TGG AG | 53.6 | ||
| F:DNA-TCG CGT CYG GTG TGA AAG | 59.4 | ||
| R:DNA-CCA CAT CCA GCR TCC AC | 55.9 | ||
| F:DNA-GGC GGC YTR CTG GGC TTT | 62.1 | ||
| R:DNA-CCA GGT GGA TWA CTT ATT GTG TTA A | 56.1 | ||
| F:DNA-AAA ACG GCA AGA AAA AGC AG | 55 | ||
| R:DNA-GCG TGG TTA CAG TCT TGC G | 58.6 | ||
| β-Actin | F:DNA-CTC CTT CCT GGG CAT GGA | 57.3 | |
| R:DNA-CGC ACT TCA TGA TCG AGT TGA | 57.8 |