Table 2. Top two single nucleotide polymorphism (SNP) markers associated with each fatty acid and possible positional candidate genes

Trait	SNP ID	Chr	Position	Allele 1	Allele 2	MAF	p-value	SNP effect	Candidate genes
C10:0	GGaluGA014205	1	40,688,299	A	G	0.02	3.34 × 10−7	0.14	ACSS3, LUM, DCN, BTG1, zDHHC17, NAV3
	Gga_rs13857775	1	43,142,234	G	A	0.02	7.12 × 10−7	0.13
C14:0	GGaluGA067637	10	6,215,383	T	C	0.29	2.41 × 10−7	0.10	MCEE, TRPM1, APBA2, FAN1, MTMR10, KLF13
	Gga_rs14003114	10	6,141,939	C	A	0.29	2.49 × 10−7	0.10
C18:0	Gga_rs14008746	10	13,643,292	C	T	0.18	1.33 × 10−6	−0.07	CGNL1, POLR2M, TCF12, ISG20, NTRK3
	Gga_rs15572763	10	7,785,707	A	C	0.22	1.47 × 10−6	−0.06
C18:1n-7	GGaluGA265712	4	74,285,145	A	G	0.39	8.50 × 10−7	−0.08	PPARGC1A, ADGRA3
	GGaluGA265714	4	74,314,272	G	A	0.39	1.00 × 10−6	−0.08
C18:1n-9	GGaluGA067637	10	6,215,383	T	C	0.29	2.40 × 10−7	0.15	MCEE, TRPM1, APBA2, FAN1, MTMR10, KLF13, ICE2
	Gga_rs14002786	10	5,518,290	G	A	0.14	2.52 × 10−7	0.21
C18:2n-6	Gga_rs14432039	4	14,106,048	T	C	0.15	1.00 × 10−6	0.02	ACSL4, ATG4A, PSMD10, NXT2, TMEM164, KCNE5
C20:0	GGaluGA070911	10	14,573,410	G	A	0.35	1.01 × 10−6	−0.06	SV2B, KLHL25, MYO5A, LYSMD2, MAPK4
	GGaluGA068440	10	9,134,046	C	T	0.12	2.44 × 10−6	0.09
C20:2	Gga_rs14380944	3	77,374,297	A	G	0.44	9.87 × 10−9	0.07	ELOVL4, CYB5R4, ME1, UBE3D, SNX14, RIPPLY2
	Gga_rs14381780	3	78,062,830	C	T	0.49	1.40 × 10−8	−0.06
C20:3n-6	Gga_rs14381780	3	78,062,830	C	T	0.49	7.24 × 10−7	−0.05	CYB5R4, ME1, PRSS35, SNAP91, PGM3, TPBG
C20:4n-6	GGaluGA067637	10	6,215,383	T	C	0.29	4.75 × 10−7	−1.44	MCEE, TRPM1, APBA2, FAN1, MTMR10, KLF13
	Gga_rs14003114	10	6,141,939	C	A	0.29	6.59 × 10−7	−1.42
C20:5n-3	Gga_rs14008746	10	13,643,292	C	T	0.18	6.23 × 10−7	−0.02	ISG20, NTRK3, MRPL46, AGBL1, RLBP1
C24:0	GGaluGA070911	10	14,573,410	G	A	0.35	3.73 × 10−7	−0.10	SV2B, ST8SIA2, MCTP2, KLHL25, AKAP13
	GGaluGA070596	10	13,926,907	C	T	0.43	6.58 × 10−7	0.10
C24:1n-9	Gga_rs15572763	10	7,785,707	A	C	0.22	3.51 × 10−7	−0.10	MNS1, RFX7, PYGO1, RAB27A
	GGaluGA068130	10	7,945,212	G	A	0.22	4.75 × 10−7	−0.10
MUFA	Gga_rs14002786	10	5,518,290	G	A	0.14	2.04 × 10−7	0.08	MCEE, TRPM1, FAN1, APBA2
	GGaluGA067478	10	5,722,494	T	C	0.14	2.04 × 10−7	0.08
PUFA	GGaluGA067637	10	6,215,383	T	C	0.29	7.75 × 10−7	−122.31	MCEE, TRPM1, APBA2, FAN1, MTMR10, KLF13
	Gga_rs14003114	10	6,141,939	C	A	0.29	1.04 × 10−6	−120.87

Chr, chromosome; Allele 1, minor allele; Allele 2, major allele; MAF, minor allele frequency; MUFA, total monounsaturated fatty acids; PUFA, total polyunsaturated fatty acids.