Temperature (T) | TN | HS | SEM | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ME (kcal/kg) (E) | 3,300 | 3,300 | 3,450 | 3,300 | HS | ||||||||
Fiber level (F) | Low | High | Low | High | Low | High | T | F | T × F | E | F | E × F | |
Phase 1 (d 0–28) (µmol/g) | |||||||||||||
Acetate | 68.89 | 75.68 | 55.69 | 60.21 | 53.9 | 59.54 | 0.93 | < 0.001 | 0.027 | 0.622 | 0.514 | 0.011 | 0.763 |
Propionate | 13.91 | 15.11 | 11.67 | 12.47 | 11.47 | 13.07 | 0.28 | < 0.001 | 0.041 | 0.665 | 0.722 | 0.041 | 0.489 |
Butyrate | 7.62 | 9.14 | 5.87 | 6.67 | 6.00 | 6.73 | 0.16 | < 0.001 | < 0.001 | 0.099 | 0.775 | 0.026 | 0.918 |
Isobutyrate | 3.40 | 2.35 | 3.30 | 3.41 | 3.15 | 3.09 | 0.14 | 0.106 | 0.109 | 0.045 | 0.420 | 0.922 | 0.783 |
Valerate | 3.26 | 3.08 | 2.92 | 2.85 | 2.81 | 2.84 | 0.07 | 0.038 | 0.331 | 0.664 | 0.713 | 0.883 | 0.726 |
Isovalerate | 4.77 | 4.89 | 4.63 | 4.14 | 4.25 | 4.21 | 0.12 | 0.117 | 0.491 | 0.259 | 0.540 | 0.293 | 0.379 |
Total SCFA | 104.4 | 112.36 | 85.07 | 92.18 | 82.65 | 89.48 | 1.42 | < 0.001 | 0.023 | 0.607 | 0.375 | 0.021 | 0.960 |
Phase 2 (d 29–56) (µmol/g) | |||||||||||||
Acetate | 80.38 | 84.54 | 60.76 | 63.36 | 60.09 | 63.73 | 0.64 | < 0.001 | 0.020 | 0.548 | 0.908 | 0.022 | 0.686 |
Propionate | 15.26 | 16.96 | 12.19 | 13.74 | 12.67 | 13.67 | 0.27 | < 0.001 | 0.008 | 0.887 | 0.704 | 0.027 | 0.622 |
Butyrate | 8.75 | 10.37 | 6.04 | 7.08 | 6.02 | 7.10 | 0.22 | < 0.001 | 0.004 | 0.454 | 0.996 | 0.027 | 0.973 |
Isobutyrate | 2.57 | 2.57 | 3.62 | 3.55 | 3.61 | 3.51 | 0.01 | < 0.001 | 0.457 | 0.485 | 0.411 | 0.006 | 0.728 |
Valerate | 3.72 | 3.48 | 2.99 | 3.25 | 2.95 | 3.13 | 0.04 | < 0.001 | 0.920 | 0.013 | 0.422 | 0.034 | 0.687 |
Isovalerate | 6.10 | 5.49 | 4.26 | 4.54 | 4.23 | 4.77 | 0.09 | < 0.001 | 0.465 | 0.061 | 0.616 | 0.045 | 0.532 |
Total SCFA | 118.1 | 124.6 | 89.91 | 95.69 | 89.88 | 96.32 | 0.91 | < 0.001 | 0.018 | 0.465 | 0.948 | 0.026 | 0.572 |