Table 1. Experimental design of Holstein steers

Treatment	No. of steers	22 month		24 month
		Concentrate		Concentrate
		Fattening	Finishing	Fattening	Finishing
Total digestible nutrients (%)1)
T1	10	70	74	-	-
T2	10	72	76	-	-
T3	10	-	-	70	74
T4	10	-	-	72	76

Each group were set to 70% (T1) and 72% (T2) during fattening phase and 74% (T3) and 76% (T4) for the finishing phase, respectively.