Table 9. Effect of energy feeding levels on physicochemical characteristics of longissimus dorsi muscle in Holstein steers

Item	22 month		24 month		SEM	p-value¹⁾
Item	T1²⁾	T2	T3	T4	SEM	p-value¹⁾
Moisture (%)	66.79	66.32	66.20	66.12	0.74	0.4513
Crude fat (%)	10.52	11.86	11.02	11.96	0.85	0.1952
Crude protein (%)	21.03	20.03	20.77	20.11	0.31	0.2398
CIE value
L*	39.77	41.35	40.72	40.12	1.17	0.1289
a*	21.07	20.98	20.88	20.34	0.73	0.4215
b*	9.52	9.02	9.52	8.99	0.38	0.2187
Chroma	22.37	23.01	23.52	23.77	0.89	0.4874
hue	24.44	25.01	24.33	24.77	0.67	0.1528

Probability of the F test.
Each group were set to 70% (T1) and 72% (T2) during fattening phase and 74% (T3) and 76% (T4) for the finishing phase, respectively.
Means in the same row with different superscripts are significantly (p < 0.05) different.