Table 2. Two-way ANOVA statistical analysis results (interaction effect)

Items	p-value
	Temperature	Oxygen	Tem × Oxy
Total cell number	0.0062	0.7237	0.3966
Live cell number	0.0055	0.7879	0.2611
Dead cell number	0.0610	0.3522	0.1283
Nuclei number	< .0001	0.1150	< .0001
MTS absorbance	< .0001	0.0052	0.6305
Myotube area	0.0764	0.0450	0.0247
Fusion index	0.0733	0.5495	0.0605
Myh2 (Gene)	< .0001	0.0731	0.1119
Myod1 (Gene)	0.3449	0.0233	0.8687
Myog (Gene)	0.0274	0.0001	0.1110
Mb (Gene)	< .0001	0.2587	0.7152
Myh (Protein)	< .0001	0.5140	0.3336
Myod1 (Protein)	< .0001	0.0779	0.0004
Myog (Protein)	< .0001	<.0001	<.0001
Mb (Protein)	< .0001	0.2587	0.7152

MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium.