Table 1. DPPH radical scavenging ability of POEE
| Extraction solvent | Concentration (ppm) | DPPH scavenging activity (%) | IC50 |
| 0−2 h | 24 h | 48 h | 50−60 h |
| 30% EtOH | 2,000 | 8.8 ± 1.7 | 12.5 ± 0.2 | 4.1 ±2.4 | 25.5 ± 12.6 | IC50 of hatching= 5,830 |
| 3,000 | 14.0 ± 1.3 | 12.9 ± 2.3 | 4.1 ± 1.9 | 27.0 ± 1.8 |
| 4,000 | 23.3 ± 1.8 | 14.6 ± 0.9 | 12.5 ± 0.3 | 39.6 ± 2.6 |
| 5,000 | 24.2 ± 0.5 | 20.9 ± 6.0 | 15.4 ± 1.6 | 46.6 ± 3.4 |
| 6,000 | 28.8 ± 0.8 | 22.1 ± 4.6 | 17.7 ± 1.2 | 55.1 ± 3.4 |
| 7,000 | 38.8 ± 2.3 | 34.8 ± 9.8 | 21.8 ± 0.9 | 62.7 ± 0.3 |
| 8,000 | 34.9 ± 2.3 | 35.3 ± 13.5 | 22.0 ± 1.1 | 60.4 ± 5.3 |
| 9,000 | 31.9 ± 9.5a | 33.3 ± 6.0a | 27.0 ± 0.7a | 71.6 ± 1.5b |
| 3rd DW | 2,000 | 4.8 ± 0.4 | 16.9 ± 2.0 | 7.8 ± 2.6 | 19.9 ± 2.3 | IC50 of hatching= 6,143 |
| 3,000 | 7.0 ± 1.0 | 22.4 ± 2.1 | 12.9 ± 5.1 | 28.7 ± 3.9 |
| 4,000 | 15.0 ± 2.6 | 31.5 ± 5.2 | 19.4 ± 5.8 | 37.3 ± 3.7 |
| 5,000 | 18.0 ± 6.5 | 35.3 ± 11.5 | 22.1 ± 8.1 | 47.6 ± 5.9 |
| 6,000 | 21.0 ± 6.8 | 39.5 ± 5.9 | 28.8 ± 8.2 | 52.7 ± 5.9 |
| 7,000 | 26.2 ± 7.7 | 47.7 ± 5.7 | 34.3 ± 8.2 | 57.7 ± 3.0 |
| 8,000 | 30.4 ± 9.8 | 46.9 ± 4.9 | 33.1 ± 9.3 | 57.1 ± 6.3 |
| 9,000 | 36.5 ± 13.8 | 53.5 ± 8.6 | 39.5 ± 11.0 | 67.3 ± 5.2 |
Superscripts indicate statistically significant differences (p < 0.05).
DPPH, 2,2-diphenyl-1-picrylhydrazyl; POEE, Paralichthys olivaceus egg extract; EtOH, ethanol; DW, distilled water.