Table 1. List of primers designed for quantitative real-time polymerase chain reaction

Primer	Forward (F)	Reverse (R)	Annealing temperature (°C)
GAPDH	5’ - GAG GGT AGT GAA GGC TGC TG - 3’	5’ - ACC AGG AAA CAA GCT TGA CG - 3’	58.0
PAX3	5’ - AGC AAC TGG AAG AGC TGG AAA G -3’	5’ - CTC CTG GGA TCA GGT GGT TAA A - 3’	64.0
PAX7	5’ - AGC TGG CAG AGA TGG AGT TG - 3’	5’ - CTA GTG GTG GTG GTG GCA AA - 3’	64.0
MYF5	5’ - AGG AGG CTG AAG AAA GTG AAC C -3’	5’ - TAG TTC TCC ACC TGT TCC CTC A - 3’	60.0
MYOD1	5’ - CAG CTA CTA CAC AGA ATC ACC AA - 3’	5’ - TCC CTT CAG CAA CAG CTT CA - 3’	63.2
MYOG	5’ - TGC CCA AGG TGG AGA TCC TA - 3’	5’ - GGG TTG GTG CCA AAC TCC AG - 3’	63.2
MSTN	5’ - GGT ATC TGG CAG AGT ATT GAT GTG AA - 3’	5’ - CAA AAT CTC TGC GGG ACC GT - 3’	57.4

GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PAX, paired box; MYF5, myogenic factor 5; MYOD1, myogenic differentiation 1; MYOG, myogenin; MSTN, myostatin.