Journal of Animal Science and Technology
Korean Society of Animal Science and Technology
Article

Association of functional sequence variants of the MYH3 gene with muscle collagen content in pigs

Yong-Jun Kang1,5, Sang-Hyun Han2, Sang-Geum Kim1,5, Su-Yeon Kim1, Hyeon-Ah Kim1,5, Yoo-Kyung Kim1, Moon-Cheol Shin1,5, Ji-Hyun Yoo1, Byoung-Chul Yang1,5, Hee-Bok Park3, Jun Heon Lee4,5,*, In-Cheol Cho1,5,**
1Subtropical Livestock Research Institute, National Institute of Animal Science, RDA, Jeju 63242, Korea.
2Habitat Conservation Division, Korean National Park Research Institute, Korea National Park Service, Yongju 36015, Korea.
3Department of Animal Resources Science, College of Industrial Sciences, Kongju National University, Kongju 32439, Korea.
4Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea.
5Reserch Association for Nanchukmacdon, Jeju 63021, Korea.
**Corresponding Author: Jun Heon Lee, Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea, Republic of. Reserch Association for Nanchukmacdon, Jeju 63021, Korea, Republic of. Phone: +82-42-821-7031. E-mail: junheon@cnu.ac.kr.
**Corresponding Author: In-Cheol Cho, Subtropical Livestock Research Institute, National Institute of Animal Science, RDA, Jeju 63242, Korea, Republic of. Reserch Association for Nanchukmacdon, Jeju 63021, Korea, Republic of. Phone: +82-64-754-5716. E-mail: choic4753@korea.kr.

© Copyright 2023 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Oct 14, 2022; Revised: Dec 31, 2022; Accepted: Jan 09, 2023

Published Online: Jan 18, 2023

Abstract

This study examined the association between functional sequence variants (FSVs) of <italic>myosin heavy chain 3</italic> (<italic>MYH3</italic>) genotypes and collagen content in a Landrace and Jeju native pig (JNP) crossbred population. Four muscles (<italic>M</italic>.<italic> longissimus dorsi</italic>, <italic>M</italic>. <italic>semimembranosus</italic>, <italic>M</italic>. <italic>triceps brachii,</italic> and <italic>M</italic>. <italic>biceps femoris</italic>) were used for the analysis of meat collagen content, and the same animals were genotyped for the FSVs of the <italic>MYH3</italic> gene by using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Three FSVs of <italic>MYH3</italic> genotypes were identified and had genotype frequencies of 0.358, 0.551, and 0.091 for <italic>QQ,</italic> <italic>Qq,</italic> and <italic>qq</italic>, respectively. <italic>QQ</italic> animals for the FSVs of the <italic>MYH3</italic> genotypes showed higher collagen content in their <italic>M</italic>. <italic>longissimus dorsi </italic>(<italic>p</italic>&lt;0.001), <italic>M</italic>. <italic>semimembranosus</italic> (<italic>p</italic>&lt;0.001), <italic>M</italic>. <italic>triceps brachii </italic>(<italic>p</italic>&lt;0.001), and <italic>M</italic>. <italic>biceps femoris</italic> (<italic>p</italic>&lt;0.001) than <italic>qq</italic> homozygous animals. After the validation of this result in other independent populations, the FSVs of <italic>MYH3</italic> genotypes can be a valuable genetic marker for improving collagen content in porcine muscles and can also be applied to increase the amount of collagen for biomedical purposes.

Keywords: Collagen content; Genotype; Muscle; MYH3; Pigs