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Journal of Animal Science and Technology
Korean Society of Animal Science and Technology

J Anim Sci Technol

pISSN: 2055-0391, eISSN: 2672-0191

DOI: https://doi.org/10.5187/jast.2023.e15

Article

Dietary supplementation of solubles from shredded, steam-exploded pine particles modulates cecal microbiome composition in broiler chickens

Chris Major Ncho1,2, Akshat Goel1,2, Vaishali Gupta2,3, Chae-Mi Jeong2,3, Ji-Young Jung4, Si-Young Ha4, Jae-Kyung Yang4, Yang-Ho Choi1,2,3,*
1Department of Animal Science, Gyeongsang National University, Jinju 52828, Korea.
2Institute of Agriculture and Life Sciences, Gyeongsang National University, jinju 52828, Korea.
3Division of Applied Life Sciences (BK21 Plus Program), Gyeongsang National University, jinju 52828, Korea.
4Department of Environmental Materials Science, Gyeongsang National University, jinju 52828, Korea.
*Corresponding Author: Yang-Ho Choi, Department of Animal Science, Gyeongsang National University, Jinju 52828, Korea, Republic of. Institute of Agriculture and Life Sciences, Gyeongsang National University, jinju 52828, Korea, Republic of. Division of Applied Life Sciences (BK21 Plus Program), Gyeongsang National University, jinju 52828, Korea, Republic of. E-mail: yhchoi@gnu.ac.kr.

© Copyright 2023 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Oct 07, 2022; Revised: Nov 28, 2022; Accepted: Feb 01, 2023

Published Online: Feb 09, 2023

Abstract

This study evaluated the effects of supplementing solubles from shredded, steam-exploded pine particles (SSPP) on growth performances, plasma biochemicals, and microbial composition in broilers. The birds were reared for 28 days and fed basal diets with or without the inclusion of SSPP from 8 days old. There were a total of three dietary treatments supplemented with 0% (0% SSPP), 0.1% (0.1% SSPP) and 0.4% (0.4% SSPP) SSPP in basal diets. Supplementation of SSPP did not significantly affect growth or plasma biochemicals, but there was a clear indication of diet-induced microbial shifts. Beta-diversity analysis revealed SSPP supplementation-related clustering (ANOSIM: R = 0.31, <italic>p </italic>&lt; 0.01), with an overall lower (PERMDISP: <italic>p</italic> &lt; 0.05) individual dispersion in comparison to the control group. In addition, the proportions of the <italic>Bacteroides</italic> were increased, and the relative abundances of the families <italic>Vallitaleaceae, Defluviitaleaceae, Clostridiaceae</italic>, and the genera <italic>Butyricicoccus</italic> and <italic>Anaerofilum</italic> (p &lt; 0.05) were significantly higher in the 0.4% SSPP group than in the control group. Furthermore, the linear discriminant analysis effect size (LEfSe) also showed that beneficial bacteria such as <italic>Ruminococcus albus</italic> and <italic>Butyricicoccus pullicaecorum</italic> were identified as microbial biomarkers of dietary SSPP inclusion (<italic>p</italic> &lt; 0.05; | LDA effect size | &gt; 2.0). Finally, network analysis showed that strong positive correlations were established among microbial species belonging to the class <italic>Clostridia</italic>, whereas <italic>Erysipelotrichia</italic> and <italic>Bacteroidia</italic> were mostly negatively correlated with <italic>Clostridia</italic>. Taken together, the results suggested that SSPP supplementation modulates the cecal microbial composition of broilers toward a “healthier” profile.

Keywords: solubles from shredded, steam-exploded pine particles; broiler; microbiome; cecum; growth