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Journal of Animal Science and Technology
Korean Society of Animal Science and Technology

J Anim Sci Technol

pISSN: 2672-0191, eISSN: 2055-0391

DOI: https://doi.org/10.5187/jast.2500035

Article

Functional properties of specific peptides derived from whey protein fermented with lactic acid bacteria isolated from dog feces

Ha-Neul Kim1, Ji-Won Lee1, Sang-Jae Lee1, Jeong-Nam Kim2, Hong-Seog Kim2, Cheol-Hyun Kim1,*
1Dandae-ro, Dongnam-gu, cheonan-si, Chungcheongnam-do, Republic of Korea, College of life science and resources 120-01, Cheonan-si 31116, Korea.
2Youdam Co., Ltd., 65 Namsan 2-gil, Jiksan-eup, Seobuk-gu, Cheonan-si 31066, Korea.
*Corresponding Author: Cheol-Hyun Kim, Dandae-ro, Dongnam-gu, cheonan-si, Chungcheongnam-do, Republic of Korea, College of life science and resources 120-01, Cheonan-si 31116, Korea, Republic of. E-mail: hichkim@dankook.ac.kr.

© Copyright 2025 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Feb 04, 2025; Revised: May 13, 2025; Accepted: May 22, 2025

Published Online: Jun 30, 2025

Abstract

To prevent degenerative joint diseases in dogs, the development of fermented whey peptides that enhance calcium absorption is essential. This study examined the physiological traits of lactic acid bacteria (LAB) derived from canine feces. It also analyzed the functional properties of the whey peptides fermented by these bacteria. Three lactic acid bacterial strains (<italic>Lactobacillus acidophilus</italic> D5, D6, and <italic>Lactobacillus plantarum</italic> D7) were selected, and the degree of whey protein hydrolysis was measured following fermentation. Among these strains, <italic>L. plantarum</italic> D7 exhibited the highest hydrolysis rate. Antioxidant and anti-inflammatory activities, along with calcium solubility, were evaluated. Fraction 2 (F2) displayed significant antioxidant activity and effectively inhibited pro-inflammatory cytokines. Additionally, 17 amino acids, including leucine, were identified. These findings suggest that <italic>L. plantarum</italic> D7 has the potential to be used as a functional food ingredient to promote canine health.

Keywords: whey protein; bioactive peptide; calcium solubilization; companion dog; lactic acid bacteria