Journal of Animal Science and Technology
Korean Society of Animal Science and Technology
Article

Combined Effects of Chitosan and Chestnut Shell on Microbial Safety and Quality of Pork Meat Batter as Potassium Sorbate Alternatives

Rina Yu1, Yejin Kim1,2, Prabhathma Rathnayake1, Heeyoung Lee2, Yun-Sang Choi3, Hae In Yong1,*
1Department of Animal Science & Biotechnology, Chungnam National University, Daejeon 34134, Korea.
2Research Group of Food Processing, Korea Food Research Institute, Wanju 55365, Korea.
3Food Standard Research Center, Korea Food Research Institute, Wanju 55365, Korea.
*Corresponding Author: Hae In Yong, Department of Animal Science & Biotechnology, Chungnam National University, Daejeon 34134, Korea, Republic of. Phone: +821027978981. E-mail: yonghaein@cnu.ac.kr.

© Copyright 2025 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Aug 27, 2025; Revised: Oct 03, 2025; Accepted: Oct 03, 2025

Published Online: Nov 03, 2025

Abstract

In the study, we aimed to evaluate the effects of chitosan and chestnut shell, individually or in combination, as alternatives to sorbate addition on the microbial safety and quality of pork meat batter. We prepared meat batters without an additive (control) and with potassium sorbate 2,000 ppm, chitosan 1%, chestnut shell 1%, and chitosan 0.5% + chestnut shell 0.5% were prepared, respectively, and stored at 4°C for 7 days. In all storage days, total aerobic bacteria, <italic>Pseudomonas </italic>spp. and lactic acid bacteria were not detected in the meat batter added with chitosan 0.5% + chestnut shell 0.5%. On storage day 7, the counts of <italic>S</italic><italic>taphylococcus</italic><italic> aureus </italic>and <italic>Escherichia coli </italic>O157:H7 were the lowest in the meat batter added with chitosan 1%, followed by that added with chitosan 0.5% + chestnut shell 0.5% (<italic>p</italic> &lt; 0.05). Thiobarbituric acid reactive substances (TBARS) value of meat batter was lowest with the addition of 1% chestnut shell, whereas the addition of chitosan 0.5% + chestnut shell 0.5% resulted in the next lowest value (<italic>p</italic> &lt; 0.05). Cooking loss was significantly higher in meat batter added with sorbate, chitosan 1%, and chitosan 0.5% + chestnut shell 0.5%, compared to the control (<italic>p</italic> &lt; 0.05). In conclusion, the combined addition of chitosan 0.5% and chestnut shell 0.5% to meat batter can be used as a sorbate substitute to prevent microbial growth and changes in quality. However, further studies are required to verify these effects in sausages and to assess additional quality changes for industrial applications.

Keywords: Meat batter; Natural substance; Chitosan; Chestnut shell; Preservative; Sorbate