MicroRNA expression profiling in the lungs of genetically different Ri chicken lines against the highly pathogenic avian influenza H5N1 virus

Sooyeon Lee1, Suyeon Kang1, Jubi Heo1, Yeojin Hong1, Thi Hao Vu1, Anh Duc Truong2, Hyun Lillehoj3, Yeong Ho Hong1,*
Author Information & Copyright
1Chung-Ang University, Anseong 17546, Korea.
2National Institute of Veterinary Research, Hanoi 100000, Viet Nam.
3USDA-ARS, Beltsville 20705, United States.
*Corresponding Author: Yeong Ho Hong, Chung-Ang University, Anseong 17546, Korea, Republic of. Phone: +82316703025. E-mail:

© Copyright 2023 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


The highly pathogenic avian influenza (HPAI) virus triggers infectious diseases, resulting in pulmonary damage and high mortality in domestic poultry worldwide. This study aimed to analyze miRNA expression profiles after infection with the HPAI H5N1 virus in resistant and susceptible lines of Ri chickens.For this purpose, resistant and susceptible lines of Vietnamese Ri chicken were used based on the A/G allele of <italic>Mx</italic> and <italic>BF2</italic> genes. These genes are responsible for innate antiviral activity and were selected to determine differentially expressed (DE) miRNAs in HPAI-infected chicken lines using small RNA sequencing. A total of 44 miRNAs were differentially expressed after 3 days of infection with the H5N1 virus. Computational program analysis indicated the candidate target genes for differentially expressed (DE) miRNAs to possess significant functions related to cytokines, chemokines, MAPK signaling pathway, ErBb signaling pathway, and Wnt signaling pathway. Several DE miRNA-mRNA matches were suggested to play crucial roles in mediating immune functions against viral evasion. These results revealed the potential regulatory roles of miRNAs in the immune response of the two Ri chicken lines against HPAI H5N1 virus infection in the lungs.

Keywords: chicken; Differentially expressed miRNAs; Highly pathogenic avian influenza; H5N1; Lung