Article

Intestinal segment and vitamin D3 concentration affect gene expression levels of calcium and phosphorus transporters in broiler chickens

Jincheng Han1,2,*, Lihua Wu1,2,3, Xianliang Lv1,2,3, Mengyuan Liu1,2,3, Yan Zhang1,2,3, Lei He1,2,4, Junfang Hao1,2, Li Xi1,2,**, Hongxia Qu1,2, Chuanxin Shi1,2, Zhiqiang Li1,2, Zhixiang Wang3, Fei Tang5, Yingying Qiao6
Author Information & Copyright
1Shangqiu Normal University, Shangqiu 476000, China.
2Henan Engineering Research Center of Green Feed Additive Development and Application, Shangqiu 476000, China.
3Henan Agricultural University, Zhengzhou 450001, China.
4Henan Normal University, Xinxiang 453007, China.
5Shandong Haineng Bioengineering Co., Ltd., Rizhao 276800, China.
6Sumy National Agrarian University, Sumy 19500, Ukraine.
**Corresponding Author: Jincheng Han, Shangqiu Normal University, Shangqiu 476000, China. Henan Engineering Research Center of Green Feed Additive Development and Application, Shangqiu 476000, China. E-mail: j.c.han@hotmail.com.
**Corresponding Author: Li Xi, Shangqiu Normal University, Shangqiu 476000, China. Henan Engineering Research Center of Green Feed Additive Development and Application, Shangqiu 476000, China. E-mail: xili_0808@163.com.

© Copyright 2022 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Aug 09, 2022; Revised: Sep 06, 2022; Accepted: Sep 16, 2022

Published Online: Oct 21, 2022

Abstract

Two experiments were conducted in this research. Experiment 1 investigated the spatial expression characteristics of calcium (Ca) and phosphorus (P) transporters in the duodenum, jejunum, and ileum of 21-day-old broilers provided with adequate nutrient feed. Experiment 2 evaluated the effects of dietary vitamin D<sub>3</sub> (VD<sub>3</sub>) concentration (0, 125, 250, 500, 1000, and 2000 IU/kg) on growth performance, bone development, and gene expression levels of intestinal Ca and P transporters in 1–21-day-old broilers provided with the negative control diet without supplemental VD<sub>3</sub>. Results in experiment 1 showed that the mRNA levels of calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), plasma membrane calcium ATPase 1b (PMCA1b), and IIb sodium-phosphate cotransporter (NaPi-IIb) were the highest in the broiler duodenum. By contrast, the mRNA levels of inorganic phosphate transporter 1 (PiT-1) and 2 (PiT-2) were the highest in the ileum. Results in experiment 2 showed that adding 125 IU/kg VD<sub>3</sub> increased body weight gain (BWG), feed intake (FI), bone weight, and percentage and weight of Ca and P in the tibia and femur of 1–21-day-old broilers compared with the negative control diet (<italic>p</italic> &lt; 0.05). The rise in dietary VD<sub>3</sub> levels from 125 to 1000 IU/kg further increased the BWG, FI, and weights of the bone, ash, Ca, and P (<italic>p</italic> &lt; 0.05). No difference in growth rate and leg bone quality was noted in the broilers provided with 1000 and 2000 IU/kg VD<sub>3</sub> (<italic>p</italic> &gt; 0.05). Supplementation with 125–2000 IU/kg VD<sub>3</sub> increased the mRNA abundances of intestinal Ca and P transporters to varying degrees. The mRNA level of CaBP-D28k increased by 536, 1161, and 28 folds in the duodenum, jejunum, and ileum, respectively, after adding 1000 IU/kg VD<sub>3</sub>. The mRNA levels of other Ca and P transporters (PMCA1b, NCX1, NaPi-IIb, PiT-1, and PiT-2) increased by 0.57–1.74 folds by adding 1000–2000 IU/kg VD<sub>3</sub>. These data suggest that intestinal Ca and P transporters are mainly expressed in the duodenum of broilers. Moreover, the addition of VD<sub>3</sub> stimulates the two mineral transporter transcription in broiler intestines.

Keywords: Vitamin D3; Broiler chicken; CaBP-D28k; PMCA1b; NaPi-IIb; PiT-1