Comparison Study between Single Enzyme and Multienzyme Complex in Distiller’s Dred Grains with Soluble Supplemented Diet in Broiler Chicken

Min-Jin Kwak1, Dong-Jin Ha2, Min Young Park3, Ju Young Eor1, Kwang-Youn Whang2, Younghoon Kim1,*
Author Information & Copyright
1Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Scinece, Seoul National University, Seoul 08826, Korea.
2Division of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 02841, Korea.
3Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, New York 10012, United States.
*Corresponding Author: Younghoon Kim, Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Scinece, Seoul National University, Seoul 08826, Korea, Republic of. Phone: 82-2-880-4808. E-mail:

© Copyright 2023 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Upregulation of the nutritional value of feed is the major target of various studies in the livestock industry, and dietary enzyme supplementation could aid in digesting the nondegrading nutrients of grains in feed ingredients. Distillers’ dried grains with solubles (DDGS) is a byproduct of the fermentation process in the beverage industry and can be used as a large supply source of fiber in feed. Therefore, we conducted an experiment with male broiler chickens to investigate the effect of various types of enzymes on DDGS and compare the efficacy of single enzyme and multienzyme complexes on growth performance and gut environments in broiler chickens. We used 840 1-day-old broiler chickens (Ross 308), and they were allotted into 2 by 4 dietary treatments with seven replications. One factor was dietary factor (DDGS supplementation), and the other was dietary enzyme addition (SE, 0.05% of β-mannanase; MC1, 0,10% of the mixture of xylanase, amylase, protease; MC2, 0.10% of the mixture of galactosidase, galactomannanase, xylanase, glucanase). The dietary exogenous enzyme in the DDGS-supplemented diet could improve growth performance as much as the growth of the control group, and digestibility of dry matter, crude protein, and gross energy were significantly increased by enzyme addition in groups of chicks fed DDGS-supplementation diet. Moreover, the populations of pathogenic bacteria, coliforms<italic>, </italic>and <italic>Bacteroidetes</italic> were significantly decreased by enzyme supplementation, which might lead to improved gut mucus-secreting cells and inflammatory cytokines in the jejunum. Collectively, dietary single enzyme and multienzyme complexes could improve gut environments, including intestinal immune responses and gut microbial population, and lead to improvement of growth performance in broiler chickens.

Keywords: enzyme; growth performance; nutrient digestibility; gut environement; broiler chicken; multienzyme complex