Dangguibuxue Decoction Protects Against Lipopolysaccharides-Induced Mastitis in bovine mammary epithelial cells in Vitro

Bovine mastitis (BM) is a prevalent and recurring disease in dairy farming. Danggui Buxue Decoction (DBD) possesses significant anti-inflammatory, antioxidative stress, and immune system regulatory effects. However, its application in the clinical prevention and treatment of bovine mastitis has not been explored. This study aimed to assess the effectiveness and mechanism of DBD on bovine mastitis through an in vitro model using lipopolysaccharide (LPS)-stimulated bovine mammary epithelial cells (BMECs). LPS stimulation led to increased expression of inflammatory markers (TNF-α, IL-1β, IL-6, and IL-8), damage indicators (BNBD5, GM-CSF, CCL2, and GADD-45α), and oxidative stress markers (COX-2, PPARγ, and iNOS) in the BMECs. Conversely, treatment with DBD counteracted these effects. Additionally, a lactate dehydrogenase (LDH) release assay indicated that DBD diminished the cellular damage caused by LPS. The Total antioxidant capacity (T-AOC), Glutathione (GSH), Superoxide dismutase (SOD), Malondialdehyde (MDA), Nitric Oxide (NO) content assay, and Reactive Oxygen Species (ROS) staining demonstrated that DBD mitigated cellular oxidative stress triggered by LPS. Moreover, DBD suppressed the mRNA and protein expression of TLR4 and NF-κB. These results imply that DBD exerts anti-inflammatory, wound healing, and antioxidative stress effects on BMECs following LPS exposure, suggesting its potential as an effective treatment for bovine mastitis.