Toxicity of the recombinant human hyaluronidase ALT-BC4 on embryonic development

Ji Hye Lee1, Miyoun Yoo2, Sang Mee Lee2, Soon-Jae Park2, Tae Young Kil4, Min Kyu Kim1,3,*
Author Information & Copyright
1Division of Animal and Dairy Science, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea.
2Alteogen Inc., 62, 1628beon-gil, Yuseong-daero, Yuseong-gu, Daejeon 34054, Korea.
3MK biotech Inc., 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea.
4Department of Social Welfare, Joongbu University, 201 Daehak-ro, Geumsan-gun, Chungcheongnam-do 32713, Korea.
*Corresponding Author: Min Kyu Kim, Division of Animal and Dairy Science, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea, Republic of. MK biotech Inc., 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea, Republic of. Phone: 82-42-821-5773. E-mail:

© Copyright 2021 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Dec 02, 2020; Revised: Dec 29, 2020; Accepted: Dec 29, 2020

Published Online: Jan 04, 2021


Cumulus-oocyte complexes (COCs), which contain immature oocytes, are matured in vitro for in vitro embryo production. Oocyte and cumulus cells are then separated using hyaluronidase. To date, there have only been a few reported cases of the toxic effects of hyaluronidase on porcine oocytes. The aim of this study was to compare the effects of bovine testis-derived hyaluronidase and recombinant human hyaluronidase on oocyte denudation and quality. Porcine COCs were matured for 44 h and denuded using different hyaluronidase concentrations and exposure times. Then, oocytes were activated by electrical parthenogenesis. In experiment 1, COCs were denuded using bovine-derived, ovine-derived (Hirax), and human recombinant (ALT-BC4) hyaluronidases for 10 and 20 min. In experiment 2, bovine-derived and human recombinant (ALT-BC4 and ICSI Cumulase<sup>®</sup>) hyaluronidases were used to denude the COCs for 2 and 20 min. In both experiments the oocytes were all completely denuded, and there was no degeneration. Rate of embryo development was significantly increased in group treated ALT-BC4 for 2 min and not significantly different in other treatment groups. In general it slightly decreased with longer exposure times. These results have confirmed that different sources of hyaluronidase do not have detrimental effects on the quality of porcine oocytes and suggest that the human recombinant hyaluronidase ALT-BC4 is suitable for oocyte denudation with an increased blastocyst rate.

Keywords: hyaluronidase; porcine; cumulus-oocyte complex (COC); parthenogenesis, embryonic development; embryonic development

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